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野生大豆是大豆遗传改良的重要资源。转基因大豆可能对野生大豆资源存在潜在的农业和生态风险。外源基因从抗草甘膦转基因大豆向野生大豆材料的逃逸不仅需要成功的杂交,还要依赖于杂交后代的适合度。因此野生大豆和抗草甘膦转基因大豆杂交后代的适合度分析,对评价抗草甘膦转基因逃逸引起的生态风险非常必要。在网室条件下,4个野生大豆材料和抗草甘膦转基因大豆RR能够杂交结实,获得有抗草甘膦基因杂交后代群体F1和F2(江浦野生豆-5×RR)。对杂交后代及其母本野生大豆材料的7个农艺性状进行调查,计算适合度并进行t测验分析。结果表明:在没有草甘膦的选择压力下,杂交后代在一些性状上的相对适合度高于母本野生大豆材料;江浦野生豆 －5 和 RR 杂交 F2代敏感株与抗性株在 7 个农艺性状的相对适合度上均差异不显著。
Wild soybeans are an important source of genetic improvement in soybeans. Genetically modified soybeans may pose potential agricultural and ecological risks to wild soybean resources. Escape of the exogenous gene from the glyphosate resistant transgenic soybean to the wild soybean material requires not only successful hybridization but also suitability for crossing offspring. Therefore, the analysis of fitness of wild soybean and glyphosate-resistant transgenic soybean is very necessary to evaluate the ecological risk caused by the escape of glyphosate-tolerant transgene. Under the conditions of net room, four wild soybean materials and glyphosate-resistant transgenic soybean RR were able to cross and obtain F1 and F2 (Jiangpu wild beans-5 × RR) population with cross-resistance to glyphosate. Seven agronomic traits of hybrids and their female wild soybean materials were investigated, fitness was calculated and analyzed by t test. The results showed that under the selection pressure of no glyphosate, the relative fitness of hybrid progenies in some traits was higher than that of female wild soybean. The susceptible and resistant strains of F2 generation of Jiangpu wild bean-5 and RR hybrids were in 7 The relative fitness of agronomic traits showed no significant difference.