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[目的]观察抗纤灵对人肝癌细胞株(HepG2)端粒酶活性和端粒酶RNA的影响,探讨中药抗纤灵防止肝细胞癌变的作用机制。[方法]将10×半抑制浓度(IC50),1.0×IC50,0.1×IC50抗纤灵药液加入到细胞培养液中,以0.85%氯化钠和环磷酰胺分别作阴性和阳性对照,在12、24、48、72、96 h收集细胞,并检测端粒酶的活性和端粒酶RNA的表达。[结果]抗纤灵杀伤HepG2的IC50为1 mg/ml,10×IC50为10 mg/ml,0.1×IC50为0.1 mg/ml;10×IC50浓度作用后,靶细胞端粒酶活性显示阴性,10×IC50及环磷酰胺对HepG2细胞端粒酶RNA有明显抑制作用;1.0×IC50和0.1×IC50则没有抑制作用。[结论]通过抑制端粒酶活性,抗纤灵能明显抑制HepG2生长。
[Objective] To observe the effect of Kangxiaoling on telomerase activity and telomerase RNA in human hepatocellular carcinoma cell line (HepG2), and to explore the mechanism of anti-fibrosis in preventing hepatocellular carcinoma. [Method] 10 × semi-inhibitory concentration (IC50), 1.0 × IC50,0.1 × IC50 anti-fibrinism liquid was added to the cell culture medium, 0.85% sodium chloride and cyclophosphamide were negative and positive controls, respectively Cells were harvested at 12, 24, 48, 72, 96 hours and telomerase activity and telomerase RNA expression were measured. [Result] The IC50 of HepG2 was 1 mg / ml, 10 × IC50 was 10 mg / ml and 0.1 × IC50 was 0.1 mg / ml. After 10 × IC50 concentration, the telomerase activity of target cells was negative, 10 × IC50 and cyclophosphamide on HepG2 cells telomerase RNA was significantly inhibited; 1.0 × IC50 and 0.1 × IC50 no inhibitory effect. [Conclusion] Kangxiling can obviously inhibit HepG2 growth by inhibiting telomerase activity.