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本文介绍采用单克隆抗体亲和层析柱一步纯化大肠杆菌不耐热毒素(LT)的方法,此法提取的LT,活性较好,纯度较高。纯化后的LT经免疫家兔已获得LT的特异抗血清。
This article describes the use of monoclonal antibody affinity chromatography step purification Escherichia coli heat-labile toxin (LT) method, the method of extraction of LT, better activity, higher purity. Purified LT-immunized rabbits have obtained LT specific antisera.