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目的筛选临床耐亚胺培南铜绿假单胞菌(IMPRPa),分析其耐药情况与Ⅰ类整合子的相关性进行研究。方法采用MIC法测定抗生素耐药性;用聚合酶链反应(PCR)法进行检测Ⅰ类整合子基因(intI),并测序确定,SPSS11.5软件分析基因与耐药的相关性。结果 81株IMPRPa均为多重耐药,IMPRPa和非IMPRPa的多重耐药率比较差异有统计学意义,111株铜绿假单胞菌中Ⅰ类整合子基因阳性率为69.4%(77/111),其中IMPRPa菌株中检出率为85.2%(69/81),非IMPRPa检出率为26.7%(8/30)。结论 IMPRPa对常用抗生素耐药性强,多重耐药现象十分严重,IMPRPa中Ⅰ类整合子的高携带率可能与亚胺培南耐药密切相关
Objective To screen clinical imipenem - resistant Pseudomonas aeruginosa (IMPRPa) and analyze the relationship between drug resistance and type Ⅰ integron. Methods Antibiotic resistance was measured by MIC method. The class Ⅰ integron gene (intI) was detected by polymerase chain reaction (PCR) and sequenced. SPSS11.5 software was used to analyze the relationship between gene and drug resistance. Results The 81 IMPRPa strains were all multi-drug resistant. The multi-drug resistance rates of IMPRPa and non-IMPRPa were significantly different. The positive rate of class Ⅰ integron gene in 111 Pseudomonas aeruginosa strains was 69.4% (77/111) Among them, the detection rate of IMPRPa was 85.2% (69/81) and that of non-IMPRPa was 26.7% (8/30). Conclusion IMPRPa is resistant to commonly used antibiotics and the multi-drug resistance is very serious. The high carrier rate of type I integron in IMPRPa may be closely related to resistance to imipenem