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应用扩散盒方法观察了肥大细胞的生长。W/W~v小鼠具有遗传性的肥大细胞缺陷。正常小鼠来源的培养肥大细胞被直接注射到W/W~v小鼠腹腔后可以存活生长,但是,注入扩散盒后再植入正常或W/W~v小鼠腹腔的培养肥大细胞却不能生长。由于NIH/3T3成纤维细胞在扩散盒中和肥大细胞共培养时可支持肥大细胞的生长,推测腹腔中有一种特定类型的细胞具有与NIH/3T3成纤维细胞相同的支持肥大细胞生长的生物学特性。肥大细胞的白介素-3依赖性和NIH/3T3细胞依赖性增殖程度不受扩散盒移植宿主小鼠基因类型(正常抑或W/W~v)的影响,说明以往报告的肥大细胞对肥大细胞的抑制作用是受肥大细胞间直接接触所介导的。
The proliferation of mast cells was observed using the diffusion box method. W / W ~ v mice have inherited mast cell defects. Normal mouse-derived cultured mast cells can be directly injected into the peritoneal cavity of W / W ~ v mice for viable growth. However, mast cells cultured in the peritoneal cavity of normal or W / W ~ v mice injected into the diffusion box can not grow Grow. Since NIH / 3T3 fibroblasts can support mast cell growth when co-cultured with mast cells in a diffusion cassette, it is speculated that one particular type of cell in the peritoneal cavity has the same biology that supports mast cell growth as NIH / 3T3 fibroblasts characteristic. The interleukin-3-dependent and NIH / 3T3 cell-dependent proliferation of mast cells is independent of the genotypes of the host mice (normal or W / W ~ v) in the diffusion cassette, indicating that the previously reported suppression of mast cells by mast cells The role is mediated by direct contact between mast cells.