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本研究的目的是制备伴放线放线杆菌全染色DNA探针,并评价其特异性和敏感性。抽提标准菌株Aa Y4 DNA,经~(32)P标记后制成探针,然后用斑点杂交法鉴定探针性质。结果Aa全染色体DNA探针的灵敏性为8×10~2纯菌或1ng同源DNA,探针与口腔常见菌的杂交显示仅与嗜沫嗜血杆菌有较弱的交叉反应。提示~(32)P标记Aa全染色体DNA探针的敏感性、特异性均较高,且方便实用,有临床推广应用价值。
The aim of this study was to prepare fully stained DNA probes for actinomycetemcomitans and to evaluate their specificity and sensitivity. The standard strain Aa Y4 DNA was extracted and labeled with ~ (32) P. The probe was then characterized by dot blot hybridization. Results The sensitivity of Aa whole chromosome DNA probe was 8 × 10 ~ 2 pure bacteria or 1 ng homologous DNA. The hybridization between probe and common oral bacteria showed weak cross-reaction with Haemophilus hydrophila. It is suggested that the sensitivity and specificity of ~ (32) P-labeled Aa whole chromosome DNA probe are high, and it is convenient and practical, and it has clinical application value.