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为探讨短暂局灶脑缺血后不同灌流期即刻早期基因（ＩＥＧｓ）与凋亡抑制基因在同一脑区的表达状况，采用不开颅血管腔内置线法制作鼠大脑中动脉阻塞（ＭＣＡ０）的局灶缺血再灌流模型，通过免疫组化法观察原癌基因ｃ－ｆｏｓ和ｂｃｌ－２蛋白在缺血３０ｍｉｎ再灌６ｈ和２４ｈ的表达。结果显示∶再灌６ｈ，二者在缺血同侧梨状皮层显著表达，而底节区表达很弱（Ｐ＜０．０１）；再灌２４ｈ，ｃ－ｆｏｓ蛋白在梨状皮层表达显著减弱（Ｐ＜０．０１）；ｂｃｌ－２蛋白表达仍显著（Ｐ＞０．０５），且在底节区的表达有增强（Ｐ＞０．０５）。因而推论∶ｃ－ｆｏｓ与ｂｃｌ－２蛋白的表达可能与缺血损害的内源性保护机制有关。
To investigate the expression of IEGs and apoptosis-inhibitory genes in the same brain regions after transient focal cerebral ischemia in rats, MCA0 The model of focal ischemia-reperfusion was used to observe the expression of c-fos and bcl-2 on the expression of proto-oncogene c-fos and bcl-2 at 6h and 24h after ischemia for 30min. The results showed that the expression of c-fos protein was significantly decreased in piriform cortex after reperfusion for 6h, both of which were significantly increased in ischemic ipsilateral piriform cortex but not in thalamus (P <0.01) (P <0.01). The expression of bcl-2 protein was still significant (P> 0.05), and the expression of bcl-2 protein was enhanced in the basal ganglia (P> 0.05). Therefore, it is concluded that the expression of c-fos and bcl-2 may be related to the endogenous protective mechanism of ischemic damage.