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目的探索乳酸菌抑制脂肪细胞分化及调节免疫功能的作用。方法将LGG乳酸菌(Lactobacillus rhamnosus GG,LGG)菌体与小鼠巨噬细胞样细胞系J774A.1细胞进行共培养,收集培养后的上清液以不同体积比添加至小鼠前脂肪细胞3T3-L1细胞的诱导分化剂中,诱导受试3T3-L1细胞分化,诱导结束后进行油红O染色,收集上清液和细胞检测脂联素(ADP)和肿瘤坏死因子α(TNF-α)的分泌量以及过氧化物酶体增殖物激活受体γ(PPAR-γ)的表达水平。结果 LGG乳酸菌与J774A.1细胞共培养后的上清液显著降低了受试3T3-L1细胞的油红吸光度(P<0.05),降低了其ADP分泌量(P<0.05),增加了TNF-α的分泌量(P<0.05),且明显抑制了PPAR-γ基因的表达(P<0.05)。结论 LGG乳酸菌具有通过活化巨噬细胞调节脂肪细胞的分化,间接地抑制脂肪蓄积及调整脂肪细胞功能的作用,相关作用机理值得深入研究。
Objective To explore the role of lactic acid bacteria in inhibiting adipocyte differentiation and regulating immune function. Methods Lactobacillus rhamnosus GG (LGG) was co-cultured with mouse macrophage-like cell line J774A.1. The supernatant was collected and cultured in 3T3- L1 cells were induced to differentiate. After induction, Oil Red O staining was performed. The supernatants and cells were collected for the detection of adiponectin (ADP) and tumor necrosis factor alpha (TNF-alpha) Secretion and peroxisome proliferator-activated receptor γ (PPAR-γ) expression levels. Results The supernatants of LGG lactic acid bacteria co-cultured with J774A.1 cells significantly reduced the oil red absorbance (P <0.05), decreased the secretion of ADP (P <0.05), and increased the expression of TNF- α secretion (P <0.05), and significantly inhibited the expression of PPAR-γ gene (P <0.05). Conclusion Lactobacillus of LGG has the function of regulating the differentiation of adipocytes by activating macrophages and indirectly inhibiting the accumulation of fat and regulating the function of adipocytes. The relevant mechanism of action is worth further study.