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目的 :了解不同种类的百日咳菌液致大鼠感染性脑水肿 (感脑 )模型的差异 ,以寻找一种新的感脑模型。方法 :SD大鼠随机分为生理盐水对照组 (NS) ,粗制百日咳菌液组 (PBR)和精制百日咳菌液组 (PBP)。分别使用粗制百日咳菌液和精制百日咳菌液制作感脑模型后 ,检测脑组织含水量、伊文思蓝 (EB)含量和钠钾离子含量。结果 :PBR组和PBP组大鼠脑含水量、钠离子含量和EB含量较NS组明显增高 ,钾离子含量明显降低 (P <0 .0 1)。PBR组全部大鼠左半球脑组织明显蓝染 ;而PBP组仅 6只大鼠脑组织出现蓝染。PBR组大鼠脑组织EB含量明显高于PBP组 (P <0 .0 1)。结论 :粗制百日咳菌液和精制百日咳菌液均可致大鼠感脑模型 ,前者以混合性脑水肿为主 ,后者以细胞内毒性脑水肿为主
Objective: To understand the different types of pertussis bacilli in rats induced by cerebral edema (sensory brain) differences in the model in order to find a new sense of the brain model. Methods: SD rats were randomly divided into saline control group (NS), crude pertussis bacilli (PBR) group and purified pertussis bacilli (PBP) group. After using the crude pertussis solution and the purified pertussis solution to make the brain-sensory model, the brain water content, Evans blue (EB) content and sodium-potassium content were measured. Results: The contents of water, Na + and EB in PBR group and PBP group were significantly higher than those in NS group (P <0.01). The left hemisphere of all the rats in the PBR group was significantly blue-stained, whereas in the PBP group only 6 of the rats were stained blue. The content of EB in brain of PBR group was significantly higher than PBP group (P <0.01). Conclusions: The crude pertussis bacilli and the purified pertussis bacilli can induce brain sensation in rats. The former is mainly mixed brain edema, while the latter is mainly intracellular toxic brain edema.