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目的:研究胍丁胺(Agm)对兔房室结细胞自发活动的影响及其作用机制.方法:应用玻璃微电极方法.结果:Agm不仅剂量依赖地抑制兔房室结细胞自发活动的V_(max),APA和VDD,RSF;而且延长APD_(90);idazoxan能明显抑制Agm的作用;而L-NAME不能影响Agm的作用;提高灌流液中的Ca~(2+)浓度可对抗Agm的作用;ATP-敏感性钾通道开放剂(lemakalim)可拮抗Agm延长APD_(90)的作用.结论:Agm对房室结细胞自发活动的抑制作用由咪唑啉受体和/或肾上腺素α_2-受体介导,并与Ca~(2+)内流和K~+外流减少有关.
Objective: To study the effect of agmatine on spontaneous activity of rabbit atrioventricular node cells and its mechanism of action.Methods: Agm was used in a dose-dependent manner to inhibit the spontaneous activity of rabbit atrioventricular node cells V_ ( max), APA and VDD, RSF, but also extend APD 90. Idazoxan can obviously inhibit the effect of Agm, while L-NAME can not affect the effect of Agm. Increasing Ca 2+ concentration in perfusate can antagonize Agm (P <0.05) .Conclusion: The inhibitory effect of Agm on the spontaneous activity of atrioventricular node cells was inhibited by imidazolinium receptor and / or adrenaline α_2-receptor Mediated, and Ca ~ (2 +) influx and K ~ + efflux decreased.