论文部分内容阅读
目的探讨柠檬酸杆菌对长期铅暴露小鼠致死率及外周血炎症因子的影响。方法将40只21d龄健康初断乳清洁级雄性KM小鼠随机分为4组:对照(蒸馏水)组和低(200mg/L)、中(400mg/L)、高(800mg/L)剂量乙酸铅染毒组,每组10只。采用自由饮水方式,连续染铅12周。染铅结束后,用1×109 CFU柠檬酸杆菌灌胃,观察小鼠死亡情况,计算7d死亡率;ELISA检测外周血IL-6、TNF-α、IFN-γ及IL-22水平。结果低、中、高剂量染铅组小鼠柠檬酸杆菌灌胃后7d死亡率分别为10%、40%、70%,与对照组相比,差异有统计学意义(P<0.05);对照组,低、中、高剂量组外周血IL-6分别为(1007±129)ng/L、(901±85)ng/L、(817±96)ng/L、(705±95)ng/L,TNF-α分别为(153±25)ng/L、(135±17)ng/L、(126±21)ng/L、(116±25)ng/L,IFN-γ分别为(49±13)ng/L、(34±6)ng/L、(30±8)ng/L、(28±7)ng/L,IL-22分别为(459±68)ng/L、(376±32)ng/L、(345±41)ng/L、(304±43)ng/L,与对照组相比,各剂量染铅组炎症因子的表达水平差异均有统计学意义(P<0.05);随着染铅剂量的升高,小鼠外周血外周血IL-6、TNF-α、IFN-γ、IL-22水平下降和死亡率上升更显著(P<0.05)。结论长期铅暴露可导致小鼠黏膜抗感染能力下降。
Objective To investigate the effects of Citrobacter on the lethality and inflammatory cytokines in mice exposed to chronic lead exposure. Methods Forty healthy 21-day-old healthy primary weaned KM male mice were randomly divided into 4 groups: control (distilled water) and low (200mg / L), medium (400mg / L) Lead poisoning group, 10 in each group. Adopt free drinking method, lead continuously for 12 weeks. After lead exposure, 1 × 109 CFU citrobacter was orally administered to observe the death of mice and calculate the 7-day mortality rate. ELISA was used to detect the levels of IL-6, TNF-α, IFN-γ and IL-22 in peripheral blood. Results The mortality rates of 7 days after the administration of Citrobacter were 10%, 40% and 70% respectively in low, medium and high dose lead-exposed mice, which were significantly different from those in control group (P <0.05) The levels of IL-6 in the peripheral blood of the low, middle, high dose groups were (1007 ± 129) ng / L, (901 ± 85) ng / L, (817 ± 96) ng / L, L and TNF-α were (153 ± 25) ng / L, (135 ± 17) ng / L, (126 ± 21) ng / L and (34 ± 6) ng / L, (30 ± 8) ng / L and (28 ± 7) ng / L, IL-22were 459 ± 68ng / (345 ± 41) ng / L and (304 ± 43) ng / L, respectively. Compared with the control group, there were significant differences in the expression of inflammatory cytokines in the lead exposure groups (P < 0.05). The levels of IL-6, TNF-α, IFN-γ and IL-22 in the peripheral blood of the mice increased more significantly with the lead dose increased (P <0.05). Conclusion Long-term lead exposure can lead to the decrease of mucosal anti-infective capacity in mice.