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Objective:To studytheeffectof p38MAPKtransfectionon thebiologicalcharacteristicsof ratglioma cellsC6.Methods :p38MAPKwas transfectedintoC6cellsby lipofectin.Expressionof p38MAPKin C6cells beforeand aftertransfectionwas detectedby immunocytochemistryand Western-blotanalysis.HE staining,transmissionelectronmicroscopyandflowcytometrywereusedto observethecellmorphology,adhesionandstudy thecellcycle.Results:p38MAPKexpressedin C6cellsaftertransfection.Cellbiologicalcharacteristicschanged,andapoptoticcellsemerged.Conclusion:Exogenousp38MAPKcouldinducetheapoptosisof C6cells.
Objective: To studytheeffectof p38MAPKtransfectionon thebiologicalcharacteristicsof ratglioma cellsC6.Methods: p38MAPKwas transfectedintoC6cellsby lipofectin.Expressionof p38MAPKin C6cells beforeand aftertransfectionwas detectedby immunocytochemistryand Western-blotanalysis.HE staining, transmissionelectronmicroscopyandflowcytometrywereusedto observethecellmorphology, adhesionandstudy thecellcycle.Results: p38MAPKexpressedin C6cellsaftertransfection.Cellbiologicalcharacteristicschanged, andapoptoticcellsemerged.Conclusion: Exogenousp38MAPKcouldinducetheapoptosisof C6cells.