睾酮酯的测定通常采用分光光度法,但该法对油性注射剂专一性不强,由于抑菌剂苯甲醇氧化后生成的苯甲醛及油剂本身均产生干扰,因此作者研究了HPLC法测定油剂中的睾酮酯类.HPLC条件:层析柱为Lichrosorb RP-8(250×4mm,id),流动相A为甲醇-四氢呋喃-水(82∶3∶15v/v),用于苯丙酸诺龙,以联苯作内标;流动相B为甲醇-四氢呋喃-水(90∶2∶8v/v),用于睾酮癸酸酯 The determination of testosterone esters is usually spectrophotometric, but the specificity of the method of oily injection is not strong, as the benzaldehyde generated by the bacteriostatic agent benzyl alcohol oxidation and oil itself interfere with each other, so the authors studied the determination of oil by HPLC Testosterone Esters in HPLC HPLC Conditions: The column was Lichrosorb RP-8 (250 × 4 mm, id) and the mobile phase A was methanol-tetrahydrofuran-water (82: 3: 15 v / v) Nandrolone with biphenyl as internal standard; mobile phase B was methanol-tetrahydrofuran-water (90: 2: 8 v / v) for testosterone decanoate