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应用旋毛虫幼虫冰冻切片抗原IFAT检测216份旋毛虫病人血清,抗体阳性率为90.74%,发病后第1周抗体阳性率70.59%,第2周升至91.30%,有显著差异(x ̄2=6.59,P<0.025),至第3、4周分别达95.83%和100%。丝虫、日本血吸虫及包虫病人和健康人血清均为阴性反应。华支睾吸虫、并殖吸虫及囊虫病人的假阳性率分别为3.33%、22.22%及3.33%。旋毛虫幼虫抗原片在-20℃至少可保存2.5年而不丧失活性。IFAT诊断人体旋毛虫病具有较高的敏感性和特异性及良好的重复性和稳定性。
Serological samples from 216 patients with Trichinella spiralis were detected by IFAT, the positive rate of antibody was 90.74%. The positive rate of antibody was 70.59% in the first week after the onset of infection and rose to 91.30% in the second week (X ~ 2 = 6.59, P <0.025), reaching 95.83% and 100% respectively by the 3rd and 4th week. Filariasis, Schistosoma japonicum and hydatid disease patients and healthy human serum were negative. The false positive rates of Clonorchis sinensis, Paragonimus and cysticercosis were 3.33%, 22.22% and 3.33% respectively. Trichinella larvae antigen tablets at -20 ℃ can be stored for at least 2.5 years without loss of activity. IFAT diagnosis of human trichinosis has high sensitivity and specificity and good repeatability and stability.