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[目的]分离鉴定萝卜蚜的病原真菌,并测定其对萝卜蚜的致病性。[方法]采用形态学方法进行病原真菌种类鉴定,利用孢子浴方法对萝卜蚜进行致病性测定。[结果]初生分生孢子,卵形,双囊壁,单核,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2。次生分生孢子形似初生分生孢子,大小为:长×宽=(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2。菌丝段菌丝状,直径为(10.6±0.8)μm。分生孢子梗掌状分枝,直径(10.0±0.9)μm。假囊状体不分枝,基部粗大,直径(19.2±1.7)μm,向端部渐尖,直径(8.0±0.9)μm。假根单菌丝状,基部直径(21.0±3.0)μm,底端具有规则的盘状固着器。休眠孢子未见。该菌对萝卜蚜的致死中量LD50=18.21mm2。[结论]感染萝卜蚜的昆虫病原真菌被鉴定为新蚜虫疠霉,该菌对萝卜蚜表现出较强的致病性。
[Objective] The research aimed to isolate and identify pathogenic fungi of radish aphids and determine their pathogenicity to radish aphids. [Method] Morphological methods were used to identify the pathogenic fungi. The pathogenicity of radish aphids was determined by spore bath method. [Results] Primary conidia, ovate, double - wall, mononuclear, (24.7 ± 1.4) μm × (10.7 ± 0.9) μm, L / D = 2.3 ± 0.2. Secondary conidia resembled primary conidia with the size of (length × width = (18.6 ± 2.1) μm × (13.3 ± 1.3) μm and L / D = 1.4 ± 0.2). Mycelium mycelium mycelial diameter (10.6 ± 0.8) μm. Conidiophores palmately branched, diameter (10.0 ± 0.9) μm. The fake cyst was unbranched and had a thick base with a diameter of (19.2 ± 1.7) μm and acuminate to the tip with a diameter of (8.0 ± 0.9) μm. Fake root mycelium mycelial, base diameter (21.0 ± 3.0) μm, the bottom has a regular disk-shaped fixer. No dormant spores. The bacteria on the radish aphids in the amount of LD50 = 18.21mm2. [Conclusion] The entomopathogenic fungi infected with Aphis gossypii were identified as a new strain of Aphis gossypii, which showed strong pathogenicity to radish aphids.