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为研究参芎注射液对脑缺血再灌注老龄大鼠炎症损伤的影响。将84只SD大鼠随机分为假手术组、模型组、尼莫通组、参芎注射液高、中、低剂量组。连续腹腔注射给药3 d,采用线栓法建立大鼠脑缺血再灌注模型,再灌注24 h,神经功能评分,取脑TTC染色,ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的含量,荧光实时定量PCR(RTPCR)对海马区IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量进行检测。结果,参芎注射液可使脑梗死体积减少,明显改善神经功能,参芎注射液能减少IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量,并减少IL-1β和TNF-α的含量。表明参芎注射液对大鼠缺血再灌注损伤具有明显的保护作用,其机制可能与抑制炎症因子表达有关。
In order to study the effect of Shenxiong Injection on inflammatory injury in the aged rats with cerebral ischemia-reperfusion. 84 SD rats were randomly divided into sham operation group, model group, nimotong group and Shenxiong injection high, medium and low dose groups. The rats were given intraperitoneal injection for 3 days. The cerebral ischemia-reperfusion model was established by thread occlusion. The neurological function score was measured 24 h after reperfusion. Brain TTC staining was performed. The level of tumor necrosis factor-α (TNF-α ), Interleukin-1β (IL-1β), and the expression of IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA in the hippocampus were detected by real- time quantitative PCR (RTPCR). As a result, Shenxiong injection can reduce the volume of cerebral infarction and improve the neurological function. Shen Gong injection can reduce the expression of IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA and IL-1β And TNF-α content. The results showed that Shenxiong injection had a significant protective effect on ischemia-reperfusion injury in rats, and its mechanism may be related to inhibiting the expression of inflammatory cytokines.