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目的:比较HPLC法和酶放大免疫法(EMIT)检测血样中甲氨蝶呤(MTX)浓度的相关性。方法:分别用HPLC法和EMIT法测定患者使用大剂量MTX 44 h后的血样,考察2种测定方法的区别和相关程度。结果:HPLC法和EMIT法检测的MTX血浓度差异有统计学意义(P<0.05),EMIT法测定结果较HPLC法高0.12μmol·L~(-1),通过passing-badlok回归分析2种方法具有良好的相关性(r=0.996 2)。结论:HPLC法和EMIT法测定MTX血浆药物浓度结果具有明显差异,在临床进行MTX药物浓度监测中应予以关注并作相应调整。
Objective: To compare the correlation of methotrexate (MTX) in blood samples by HPLC and Enzyme Immunoassay (EMIT). Methods: The HPLC method and EMIT method were used to determine the difference between the two methods and the correlation between the two methods. Results: The difference of MTX blood concentration between HPLC and EMIT was statistically significant (P <0.05). The results of EMIT were 0.12 μmol·L -1 higher than those of HPLC, and the two methods were analyzed by passing-badlok regression There was a good correlation (r = 0.996 2). Conclusion: There are significant differences between the HPLC and EMIT methods in the determination of MTX plasma drug concentration. Attention should be paid to the MTX drug concentration monitoring in clinic and be adjusted accordingly.