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目的:研究补肾中药对去势大鼠肾组织中骨形成蛋白-4(BMP-4)的mRNA表达的影响。方法:将雌性大鼠随机分为7组。低剂量组给予补肾中药复方,每天0.7 g/kg;中剂量组给予补肾中药复方,每天2.1 g/kg;高剂量组给予补肾中药复方,每天6.3 g/kg;骨疏康组给予骨疏康颗粒,每天2.1 g/kg;牡蛎碳酸钙组给予牡蛎碳酸钙咀嚼片,每天0.006 3 g/kg。每天灌胃1次,各组给药容积为100g体重/mL。正常组、模型组每天灌同体积的生理盐水。灌胃12周后取材并进行指标检测:用RT-PCR法和Western法检测大鼠肾组织中BMP-4的mRNA和蛋白表达。结果:大鼠肾组织可以在基因、蛋白水平表达BMP-4,绝经后骨质疏松症的发生与肾组织中BMP-4的mRNA表达的变化有关。结论:补肾中药可明显上调肾中BMP-4的蛋白表达,这可能是其防治绝经后骨质疏松症的机制之一。
Objective: To study the effects of Bushen Chinese medicine on the expression of BMP-4 mRNA in the kidney of ovariectomized rats. Methods: Female rats were randomly divided into 7 groups. The low-dose group was given Bushen Chinese compound, 0.7 g / kg daily; the middle-dose group was given Bushen Chinese compound, 2.1 g / kg daily; the high dose group was given Bushen Chinese compound, 6.3 g / kg daily; Granules, 2.1 g / kg daily; oyster calcium carbonate group orally given calcium carbonate chewable tablets, 0.006 3 g / kg daily. Gavage once a day, each group administration volume of 100g body weight / mL. Normal group, model group were injected with the same volume of saline daily. Twelve weeks after gavage, samples were taken and the indexes were detected: The mRNA and protein expression of BMP-4 in renal tissue were detected by RT-PCR and Western blot. Results: The rat renal tissue could express BMP-4 at the gene and protein levels. The incidence of postmenopausal osteoporosis was related to the change of mRNA expression of BMP-4 in the kidney. Conclusion: Bushen Chinese medicine can significantly upregulate the protein expression of BMP-4 in the kidney, which may be one of the mechanisms of prevention and treatment of postmenopausal osteoporosis.