论文部分内容阅读
目的 :研究 4 3例喉癌和 18例下咽癌术后标本中增殖细胞核抗原 (PCNA)的表达并对核仁组成区相关嗜银蛋白 (AgNOR)计数 ,分析其与临床分期、病理分化、复发、转移、生存期之间的相关性 ,评估其对预后及监测患者病情的价值。方法 :采用免疫组化法及Crocker’sAgNOR染色技术定量观察 6 1例鳞状细胞癌术后标本及9例良性喉肿瘤患者的非瘤区粘膜中PCNA的表达及AgNOR计数。分析其与喉癌及下咽癌临床分期、病理分级、复发、转移、生存期之间的相关性。结果 :PCNA阳性细胞百分率 (PCNA指数 )与AgNOR计数呈正相关 ;PC NA指数与AgNOR计数由早期到晚期 ,由高分化到低分化逐渐提高 ;两者在复发、转移及期内死亡患者的癌组织中增加 (P <0 .0 5 )。结论 :PCNA指数和AgNOR计数可作为喉及下咽鳞癌的诊断性预测指标。
Objective: To study the expression of proliferating cell nuclear antigen (PCNA) in postoperative specimens of 43 cases of laryngeal carcinoma and 18 cases of hypopharyngeal carcinoma and to count the number of AgNOR related to the nucleolar organizer area. The clinical stage, pathological differentiation, Recurrence, metastasis, survival of the correlation between the assessment of their prognosis and monitoring the value of the patient’s condition. Methods: Immunohistochemistry and Crocker ’sAgNOR staining were used to quantitatively observe the expression of PCNA and AgNOR count in mucosa of non-tumorous mucosa in 61 cases of squamous cell carcinoma specimens and 9 cases of benign laryngeal tumors. The correlation between clinical stage, pathological grade, recurrence, metastasis and survival of laryngeal and hypopharyngeal carcinoma was analyzed. Results: The PCNA positive cell number (PCNA index) was positively correlated with the AgNOR count. The PCNA index and AgNOR count increased from high differentiation to poor differentiation gradually. The recurrence, metastasis and the death of the cancer tissue (P <0.05). Conclusion: PCNA index and AgNOR count can be used as diagnostic predictors of laryngeal and squamous cell carcinoma.