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本文对固相放射免疫法检测乙型病毒性肝炎表面抗原的可重复性、灵敏性、特异性等作了初步探讨,并与Abbott药盒进行了对照。 应用马抗-HBs作包被固相抗体和标记抗体时,在标记抗体的稀释液中应加入正常马血清,可避免不同种动物之间血清的非特异性反应。 应用对流免疫电泳(CIEP)法可检出的HBsAg含量为1.29μg/ml,我室的SPRIA-HBsAg检测系统可检出的HBsAg含量为4.1ng/ml,而Abbott药盒为2.1ng/ml。我室检测系统的灵敏性为Abbott药盒的1/2。 应用上述检测系统对广州地区正常人群进行了测试,得出常数为2.21,Abbott药盒则为2.1。 HBsAg检出率在正常人群为12.9～17.7％,急性肝炎为60.6％,慢性肝炎为89.9％,说明我国人群乙型肝炎病毒感染极为常见。因此,在临床诊断、筛选献血员及流行病学等方面推广使用这一检测方法尤为重要和迫切。
In this paper, we investigated the repeatability, sensitivity and specificity of solid-phase radioimmunoassay for the detection of hepatitis B virus surface antigen and compared it with Abbott kit. When using horse anti-HBs for coating solid-phase antibody and labeled antibody, normal horse serum should be added to the diluted antibody of labeled antibody to avoid the nonspecific reaction of serum between different species. The detection of HBsAg by CIEP was 1.29μg / ml. The detection of HBsAg in our room was 4.1ng / ml by SPRIA-HBsAg detection system and 2.1ng / ml by Abbott kit. The sensitivity of our chamber detection system is 1/2 of Abbott kit. Using the above detection system to test the normal population in Guangzhou, a constant of 2.21 was obtained, while that of Abbott kit was 2.1. The detection rate of HBsAg is 12.9-17.7% in the normal population, 60.6% in acute hepatitis and 89.9% in chronic hepatitis, indicating that hepatitis B virus infection in our population is extremely common. Therefore, it is particularly important and urgent to promote the use of this method in clinical diagnosis, screening of blood donors and epidemiology.