论文部分内容阅读
目的:观察丹参酮ⅡA对高血压病血瘀证患者血清致伤CRL-1730胞浆内游离钙的影响。方法:将培养的人脐静脉内皮细胞株CRL-1730按模型组、非血瘀组、健康组、对照组、药物组(TanⅡA10μg/mL)分组,激光扫描共聚焦显微镜检测各组细胞胞浆游离钙浓度。结果:模型组与健康组比较,荧光强度较大,差异显著(P<0.05),模型组与空白对照组比较,荧光强度较大,差异显著(P<0.01);模型组与非血瘀组比较,无显著差异(P>0.05);药物组与模型组比较,荧光强度较小,但无显著差异(P>0.05)。结论:高血压病血瘀证患者血清可以损伤内皮细胞,丹参酮ⅡA对内皮细胞具有保护作用。
Objective: To observe the effect of tanshinone ⅡA on intracellular free calcium of CRL-1730 induced by serum in patients with blood-stasis syndrome of hypertension. Methods: The cultured human umbilical vein endothelial cell line CRL-1730 was divided into model group, non-blood-stasis group, healthy group, control group and drug group (TanⅡA10μg / mL). Laser scanning confocal microscopy was used to detect the cytosolic free Calcium concentration. Results: The fluorescence intensity of the model group was significantly higher than that of the healthy group (P <0.05), and the fluorescence intensity of the model group was significantly higher than that of the blank control group (P <0.01) (P> 0.05). Compared with the model group, the fluorescence intensity of the drug group was smaller, but there was no significant difference (P> 0.05). Conclusion: Serum of patients with hypertension and blood stasis can damage endothelial cells, and tanshinone Ⅱ A can protect endothelial cells.