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利用3-磺酸丙基甲基丙烯酸酯钾盐为功能单体,聚乙二醇二丙烯酸酯为交联剂,水、甲醇、环己醇为三元致孔剂及无机基质硅胶微球,在内径为530μm熔融石英毛细管内通过原位聚合的方法制备了一种新型有机聚合物包覆硅球性强阳离子交换整体柱.通过比较不同条件下制备的整体柱的通透性、压力对流速的关系曲线和扫描电镜图,确定了致孔剂溶剂组成,优化了整体柱的单体总浓度,致孔剂溶剂比例及反应时间.最终确定制备此整体柱的致孔剂水:甲醇:环己醇的体积比为1:6:11,最佳单体总浓度为25%(C=40%),最佳反应时间为180 min.该整体柱有望在多维色谱分离复杂多肽和蛋白质的应用中起到重要作用.
Using 3-sulfonic acid propylmethacrylate as functional monomer, polyethylene glycol diacrylate as crosslinking agent, water, methanol and cyclohexanol as ternary porogen and inorganic matrix silica microspheres, A new type of organic polymer coated silica-sphere strong cation exchange monolithic column was prepared by in-situ polymerization in a fused silica capillary tube with an inner diameter of 530μm. By comparing the permeability of the monolithic column prepared under different conditions, The relationship between solvent and composition of porogen and the reaction time were optimized, and the pore-forming agent for preparing the monolithic column was finally confirmed to be water: methanol: ring Hexanol volume ratio of 1: 6: 11, the best total monomer concentration of 25% (C = 40%), the optimal reaction time was 180 min. The monolithic column is expected in the multidimensional chromatographic separation of complex peptides and proteins Play an important role.