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目的研究马鞍山和苏州地区5岁以下腹泻儿童轮状病毒G型和P型的分型。方法对马鞍山和苏州地区5岁以下急性腹泻儿童粪便标本1267份,采用酶联免疫吸附试验和巢式-聚合酶链式反应检测轮状病毒感染和血清型。结果轮状病毒阳性标本378份,主要来自2岁以下婴幼儿;血清型G型是优势株共250株,其中G1100株,G28株,G3111株,G43株,G93株,混合感染4株,未能分型21株(8.40%;马鞍山以G1型为主(58.54%苏州以G3型为优势株(47.85%;P基因型两地区流行株为P4和P8,分别占52.33%和47.67%,G1P4成为仅次于G1P8的GP组合。结论在苏州和马鞍山地区5岁以下儿童轮状病毒腹泻中,流行的血清型分别为G3和G1,P基因型流行株为P4和P8。
Objective To study the genotyping of G and P type rotavirus in children under 5 years of age in Ma’anshan and Suzhou areas. Methods 1267 children with acute diarrhea under 5 years old in Ma’anshan and Suzhou areas were enrolled in this study. Enzyme - linked immunosorbent assay (ELISA) and nested polymerase chain reaction (PCR) were used to detect rotavirus infection and serotypes. Results There were 378 rotavirus positive samples, mainly from infants under 2 years old. Serotype G was 250 dominant strains, of which G1100, G28, G3111, G43, G93, mixed infection 4 There were 21 strains (8.40%) in Maanshan and 58.54% in Suzhou (47.85%). The prevalence of P and P8 genotypes in the two regions were 52.33% and 47.67%, respectively. G1P4 And became the GP combination next only to G1P8.Conclusion The prevalence of rotavirus diarrhea in children under 5 years old in Suzhou and Ma’anshan was G3 and G1, respectively, and P genotypes were P4 and P8.