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目的探究CD11b与CD27定义的T细胞亚群与HIV疾病进展的相关性,为研究HIV感染者T细胞免疫缺陷提供新的思路。方法外周血荧光抗体染色,用流式细胞仪检测CD11b、CD27及各亚群的表达;破膜胞内染色检测各亚群分泌IFN-γ的功能;细胞因子与PBMC共培养,检测CD11b、CD27的变化。结果在HIV-1感染者,CD27~+CD11b~-、CD27~+CD11b~+和CD27~-CD11b~+的T细胞亚群明显降低(P<0.05),而CD27~-CD11b~-T细胞亚群明显增多(P<0.05),该亚群与CD4~+T细胞呈显著的负相关(r=-0.545,P<0.001);CD27~+CD11b~-、CD27~+CD11b~+和CD11b~+CD27~-亚群分泌IFN-γ功能均强于CD27~-CD11b~-亚群;细胞因子TGF-β随着质量浓度的增加对CD11b表达的抑制也逐渐增强,而IL-12和IL-15能够刺激CD11b的增多,IL-2与IL-7能够刺激CD27增多。结论 HIV感染导致人体T细胞亚群发生紊乱。
Objective To explore the relationship between T cell subsets defined by CD11b and CD27 and the progression of HIV disease and to provide new ideas for the study of T cell immunodeficiency in HIV-infected individuals. Methods The expression of CD11b, CD27 and its subpopulations were detected by flow cytometry (FCM). The function of IFN-γ secreted by each subpopulation was detected by the method of flow cytometry. The cytokines were co-cultured with PBMC to detect the expression of CD11b, CD27 The change. Results The T cell subsets of CD27 ~ + CD11b ~ -, CD27 ~ + CD11b ~ + and CD27 ~ -CD11b ~ + were significantly decreased in HIV-1 infected patients (P <0.05) (R = -0.545, P <0.001); CD27 ~ + CD11b ~ -, CD27 ~ + CD11b ~ +, and CD11b were significantly negatively correlated with CD4 + T cells (P <0.05) ~ + CD27 ~ - subunit secretion of IFN-γ were stronger than CD27 ~ -CD11b ~ - subgroup; cytokinin TGF-βincreased with the increase of the concentration of CD11b expression also gradually increased, while IL-12 and IL -15 can stimulate the increase of CD11b, IL-2 and IL-7 can stimulate CD27 increased. Conclusion HIV infection leads to disorder of human T cell subsets.