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目的:研究电针结合银杏酮酯(GBE 50)对D-半乳糖致衰老大鼠学习记忆障碍和海马细胞因子含量的影响,探索针药结合治疗对学习记忆改善作用的机制。方法:SD大鼠随机分成正常对照组、模型组、电针组、银杏酮酯组和针药结合组。采用腹腔注射D-半乳糖的方法建立记忆障碍衰老大鼠模型。电针组在造模第21天开始给予电针治疗,选用“百会”、双侧“足三里”穴位,隔天1次,治疗21 d;银杏酮酯组在造模第21天开始按150 mg/kg给予GBE 50灌胃,每天1次,持续21 d;针药结合组在给予D-半乳糖腹腔注射和GBE 50灌胃后给予电针治疗。治疗结束后采用Morris水迷宫观察大鼠行为学变化以及放射免疫分析方法检测各组大鼠海马白介素(IL)-1β、IL-6和肿瘤坏死因子(TNF)-α的含量。结果:模型组与正常对照组相比逃避潜伏期明显延长,游泳距离百分比明显减少(P<0.05,P<0.01);而电针组、银杏酮酯组和针药结合组与模型组相比,逃避潜伏期均显著缩短(P<0.05,P<0.01),游泳距离百分比明显增大(P<0.01),且针药结合组逃避潜伏期与电针组和银杏酮酯组相比差异有统计学意义(P<0.05)。模型组与正常对照组相比大鼠海马IL-1β和TNF-α含量增高,IL-6含量明显降低(P<0.05,P<0.01);与模型组比较,电针组、银杏酮酯组、针药结合组大鼠海马IL-1β含量均明显降低(P<0.05,P<0.01),银杏酮酯组和针药结合组IL-6含量均显著升高(P<0.01),电针组和针药结合组TNF-α含量明显下降(P<0.05)。结论:电针和GBE 50对大鼠海马IL-1β、IL-6和TNF-α含量均有不同程度的调节作用,抑制D-半乳糖引发的中枢神经系统免疫炎性反应;针药合用在改善学习记忆方面有一定的协同作用。
Objective: To investigate the effects of electroacupuncture combined with GBE 50 on learning and memory impairment and hippocampal cytokine content in aging rats induced by D-galactose and to explore the mechanism of acupuncture and medicine in improving learning and memory. Methods: SD rats were randomly divided into normal control group, model group, electroacupuncture group, ginkgolide group and acupuncture combination group. The rat model of memory impairment was established by intraperitoneal injection of D-galactose. The electroacupuncture group received electro-acupuncture treatment on the 21st day of model making. The acupoints of “Baihui”, bilateral “Zusanli” acupoints were treated once every other day for 21 days. Beginning on the 21st day, GBE 50 was administered intragastrically at a dose of 150 mg / kg once daily for 21 days. Electro-acupuncture was given after intraperitoneal injection of D-galactose and GBE 50. Morris water maze test was used to observe the changes of behavior and radioimmunoassay in rats hippocampus to observe the levels of IL-1β, IL-6 and TNF-α in the hippocampus. Results: Compared with the normal control group, the escape latency of the model group was significantly longer and the percentage of swimming distance was significantly decreased (P <0.05, P <0.01). Compared with the model group, the EA group, Ginkgo biloba ester group and acupuncture- Escape latency (P <0.05, P <0.01), swimming distance percentage increased significantly (P <0.01), and the drug-drug combination group escape latency compared with EA group and Ginkgo biloba ester group was statistically significant (P <0.05). The levels of IL-1β and TNF-α in the hippocampus of model group were significantly higher than those in the normal control group (P <0.05, P <0.01). Compared with the model group, the levels of IL-1β and TNF- (P <0.05, P <0.01). The levels of IL-6 in ginkgolide group and acupuncture-drug combination group were significantly increased (P <0.01). The levels of electroacupuncture The level of TNF-α in the combination group was significantly decreased (P <0.05). CONCLUSION: EA and GBE50 can regulate the levels of IL-1β, IL-6 and TNF-α in hippocampus of rats to different degrees and inhibit the central nervous system immune-inflammatory reaction induced by D-galactose. Improve learning and memory have some synergy.