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目的:为了研究Fas/FasL介导的细胞凋亡在桥本氏甲状腺炎发病机制中的作用,评价其在治疗中的意义.方法:采用流式细胞仪、双抗体夹心ELISA法及免疫组化方法分别测定桥本氏甲状腺炎(HT)及正常时照组外周血T淋巴细胞Fas抗原表达情况,血浆中sFas水平及甲状腺腺泡细胞Fas抗原及Fas配体的表达情况.结果:发现HT组甲状腺细胞Fas和FasL表达强度显著高于正常对照组(P<0.01),血浆sFas水平(2.05ng/ml±0.54ng/ml)明显低于正常组(3.59ng/ml±0.68ng/ml,P<0.001),HT外周血T淋巴细胞Fas抗原阳性细胞数(79.99％±16.08％)显著高于正常对照组(57.22％±11.57％,P<0.001).结论:Fas系统介导的细胞凋亡与桥本氏甲状腺炎发病密切相关。
AIM: To investigate the role of Fas / FasL-mediated apoptosis in the pathogenesis of Hashimoto’s thyroiditis and evaluate its significance in the treatment.Methods: Flow cytometry, double antibody sandwich ELISA and immunohistochemistry Methods The expression of Fas antigen in peripheral blood T lymphocytes, the level of sFas in plasma and the expression of Fas antigen and Fas ligand in thyroid acinar cells were measured respectively in Hashimoto’s thyroiditis (HT) and normal control group.Results: The expression of Fas and FasL in thyroid cells was significantly higher than that in normal control group (P <0.01). The level of sFas in plasma (2.05ng / ml ± 0.54ng / ml) was significantly lower than that in normal group (3.59ng / ml ± 0.68ng / <0.001). The number of Fas antigen-positive cells in peripheral blood T lymphocytes (79.99% ± 16.08%) in HT group was significantly higher than that in the normal control group (57.22% ± 11.57%, P <0.001) .Conclusion: Fas- And Hashimoto’s thyroiditis is closely related to the incidence.