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目的建立同时测定珍龙醒脑胶囊中没食子酸、肉桂酸、甘草苷、西红花苷I、西红花苷II、桂皮醛、丁香酚、甘草次酸、麝香酮等9种成分的UPLC-DAD方法。方法采用RP-UPLC法,Agilent Zorbax C18色谱柱(100 mm×2.1 mm,1.8μm),流动相为0.1%甲酸水溶液-乙腈,体积流量0.3 m L/min,梯度洗脱,柱温35℃,进样量为1μL。分别对待测物考察其提取溶剂、线性范围、精密度、重复性、稳定性、加样回收率等方法学内容。结果 9种指标成分没食子酸在0.2~20.0mg/L(r=0.999 0)、西红花苷I在0.022~2.200 mg/L(r=0.999 3)、西红花苷II在0.02~2.00 mg/L(r=0.999 6)、甘草苷在0.093~1.860 mg/L(r=0.999 4)、肉桂酸在0.020 4~2.040 0 mg/L(r=0.999 9)、桂皮醛在0.042~4.200 mg/L(r=0.999 9)、丁香酚在0.95~95.00 mg/L(r=0.999 9)、甘草次酸在0.012 9~1.290 0 mg/L(r=0.998 9)、麝香酮在0.073 8~7.380 0 mg/L(r=0.999 0)线性关系良好;精密度RSD≤1.02%;重复性良好,RSD≤1.13%;加样回收率在93.8%~112.1%,RSD≤1.28%。供试品溶液在室温条件下24 h内稳定。3批次供试品中没食子酸、西红花苷I和II、甘草苷、肉桂酸、桂皮醛、丁香酚、甘草次酸、麝香酮质量分数分别为0.422~0.448、0.093~0.105、0.096~0.112、0.026 8~0.028 5、0.142~0.153、0.140~0.158、1.519~1.547、0.007 55~0.008 04、0.117~0.121 mg/g。结论本法快速、灵敏度高、准确度高、专属性好,为珍龙醒脑胶囊的质量控制提供依据。
OBJECTIVE To establish a HPLC method for the simultaneous determination of nine components of gallic acid, cinnamic acid, licorice glycoside, crocin I, crocin II, cinnamaldehyde, eugenol, glycyrrhetinic acid and muscone in Zhenlong Xingnao Capsule. DAD method. Methods The RP-UPLC method was used on an Agilent Zorbax C18 column (100 mm × 2.1 mm, 1.8 μm). The mobile phase consisted of 0.1% formic acid in water and acetonitrile, and the volume flow rate was 0.3 m L / Injection volume of 1μL. The analytes were investigated for their extraction solvent, linear range, precision, repeatability, stability, sample recovery methodological content. Results The results showed that the concentrations of gallic acid in the range of 0.2-20.0 mg / L (r = 0.999 0), cropexin I in the range of 0.022-2.200 mg / L (r = 0.999 3) and cinerase II in the range of 0.02-2.00 mg (R = 0.999 4), cinnamic acid was 0.020 4 ~ 2.040 0 mg / L (r = 0.999 9), cinnamaldehyde was between 0.042 ~ 4.200 mg / L (R = 0.999 9), glycyrrhetinic acid ranged from 0.012 9 to 1.290 0 mg / L (r = 0.998 9), and the contents of muscone at 0.073 8 ~ The linearity was 7.380 0 mg / L (r = 0.999 0). The precision RSD was less than or equal to 1.02%. The reproducibility was good with RSD≤1.13%. The recoveries were 93.8% ~ 112.1% and RSD≤1.28%. The test solution was stable within 24 h at room temperature. The mass fractions of gallic acid, crocin I and II, licorice, cinnamic acid, cinnamaldehyde, eugenol, glycyrrhetinic acid and muscone in the three batches were 0.422-0.448, 0.093-0.105, 0.112,0.026 8 ~ 0.028 5,0.142 ~ 0.153,0.140 ~ 0.158,1.519 ~ 1.547,0.007 55 ~ 0.008 04,0.117 ~ 0.121 mg / g. Conclusion This method is rapid, sensitive, accurate and specific, providing the basis for the quality control of Zhenlong Xingnao Capsule.