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目的研究气体信号分子硫化氢(H2S)对氧化型低密度脂蛋白(ox-LDL)诱导的人单核细胞白血病细胞(THP-1)来源的人巨噬细胞单核细胞趋化蛋白-1(MCP-1)分泌的影响。方法将THP-1来源的人巨噬细胞分为4组:正常对照组、ox-LDL组、ox-LDL+H2S 100组及ox-LDL+H2S 500组。正常对照组:细胞于基础培养基中培养48 h;ox-LDL组:在基础培养基中加入50 mg.L-1ox-LDL,培养48 h;ox-LDL+H2S 100组在基础培养基中先加入100μmol.L-1NaHS,孵育30 min后再加入50 mg.L-1ox-LDL,培养48 h;ox-LDL+H2S 500组在基础培养基中先加入500μmol.L-1NaHS,孵育30 min后再加入50 mg.L-1ox-LDL,培养48 h。用ELISA法检测细胞上清液中MCP-1水平。结果与正常对照组[(34.58±6.77)μmol.L-1]比较,ox-LDL组[(66.27±7.29)μmol.L-1]、ox-LDL+H2S 100组[(49.45±3.08)μmol.L-1]及ox-LDL+H2S 500组[(46.64±5.47)μmol.L-1]细胞上清液中MCP-1水平均明显升高(Pa<0.05);与ox-LDL组比较,ox-LDL+H2S 100组及ox-LDL+H2S 500组细胞上清液中MCP-1水平均明显降低(Pa<0.05)。ox-LDL+H2S 100组细胞上清液中MCP-1水平与ox-LDL+H2S 500组比较差异无统计学意义(P>0.05)。结论ox-LDL可诱导人巨噬细胞MCP-1分泌增加;给予外源性H2S的供体NaHS可明显抑制ox-LDL诱导的MCP-1分泌增加。
Objective To investigate the effects of gaseous hydrogen sulfide (H2S) on human macrophage chemotactic protein-1 (THP-1) -induced apoptosis in human monocytic leukemia cells (THP-1) induced by oxidized low density lipoprotein MCP-1) secretion. Methods THP-1-derived human macrophages were divided into 4 groups: normal control group, ox-LDL group, ox-LDL + H2S 100 group and ox-LDL + H2S 500 group. The normal control group: the cells were cultured for 48 h in the basal medium; the ox-LDL group: 50 mg.L-1ox-LDL in the basal medium, cultured for 48 h; the ox-LDL + H2S 100 group in the basal medium First, add 100μmol.L-1NaHS for 30 min, then add 50 mg.L-1ox-LDL for 48 h; ox-LDL + H2S 500 group add 500μmol.L-1NaHS to basal medium and incubate for 30 min After adding 50 mg.L-1ox-LDL, cultured for 48 h. The level of MCP-1 in the cell supernatant was measured by ELISA. Results Compared with normal control group [(34.58 ± 6.77) μmol.L-1], ox-LDL group [(66.27 ± 7.29) μmol.L-1] and ox-LDL + H2S 100 group [(49.45 ± 3.08) μmol (P <0.05). Compared with the ox-LDL group, the level of MCP-1 in ox-LDL + H2S 500 group [(46.64 ± 5.47) μmol.L-1] MCP-1 in ox-LDL + H2S 100 group and ox-LDL + H2S 500 group were significantly decreased (P <0.05). The level of MCP-1 in ox-LDL + H2S 100 cells supernatant was not significantly different from ox-LDL + H2S 500 (P> 0.05). Conclusion Ox-LDL can induce the increase of MCP-1 secretion in human macrophages. The donor of exogenous H2S can significantly inhibit the increase of MCP-1 secretion induced by ox-LDL.