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目的探讨大鼠急性羰基镍中毒各脏器谷胱甘肽过氧化物酶(GSH-Px)活力变化。方法 SD大鼠200只,分为低、中、高剂量羰基镍实验组、氯气染毒对照组和正常对照组,每组各40只。3个实验组分别给予20、135、250 mg/m3羰基镍染毒,氯气染毒对照组给予250 mg/m3氯气染毒,正常对照组未予特殊处理。静态吸入染毒,染毒时间为30 min,每组染毒1次,染毒后1、2、3和7 d取材,应用化学比色法测定肺、肝、肾、心和脾脏GSH-Px活力。结果在低、中、高剂量羰基镍染毒条件下,大鼠肺、肝、肾、心GSH-Px活力均有下降,以高剂量羰基镍实验组GSH-Px下降最显著(P<0.05);不同取材时间GSH-Px活力比较,染毒后3 d脏器GSH-Px活力下降最明显(P<0.05);氯气染毒后各脏器GSH-Px活力均显著下降(P<0.05)。结论急性羰基镍中毒可诱发组织氧化损伤,降低机体清除自由基的能力,并存在组织差异。
Objective To investigate the changes of glutathione peroxidase (GSH-Px) activity in various organs of acute carbonyl nickel poisoning in rats. Methods 200 SD rats were divided into low, medium and high doses of carbonyl nickel experimental group, chlorine exposure control group and normal control group, 40 rats in each group. 3 experimental groups were given nickel carbonyl 20,135,250 mg / m3, chlorine poisoning control group was given 250 mg / m3 chlorine poisoning, the normal control group without special treatment. Static inhalation exposure, exposure time was 30 min, each exposure time 1, 2, 3 and 7 d after exposure, the chemical colorimetric determination of lung, liver, kidney, heart and spleen GSH-Px vitality. Results The activities of GSH-Px in lung, liver, kidney and heart of rats were decreased under low, medium and high doses of nickel carbonyl treatment. The GSH-Px decreased most significantly in high-dose carbonyl nickel test group (P <0.05) (P <0.05). GSH-Px activity in different organs at different time points was significantly lower than that at 3 days (P <0.05). GSH-Px activity in various organs was significantly decreased after chlorine exposure (P <0.05). Conclusion Acute carbonyl nickel poisoning can induce tissue oxidative damage, reduce the body’s ability to scavenge free radicals, and there are tissue differences.