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松油和桂皮油由于具有芳香性气味,因而成为良好的成虫驱避剂,但是关于它们对蚊虫的杀幼虫作用研究不多。为揭示市售的长叶松Pinus longifolia油和锡兰肉桂Cinnamomum zeylanicum油对来源于印度德里的埃及伊蚊Aedes aegypti4龄幼虫的毒杀潜力,我们进行了室内研究,以幼虫死亡率及行为改变和形态改变等指标评估其杀幼虫潜力。结果表明:两种油对埃及伊蚊4龄幼虫均具有毒杀作用,且松油的灭幼效果优于桂皮油。松油的LC50和LC70值分别为0.33093mg/L和0.54476mg/L,而桂皮油的LC50和LC70值分别为0.63159mg/L和0.77736。进一步观察发现,LC90剂量下桂皮油的杀幼虫潜力强于松油,其LC90为1.11879mg/L,而松油的LC90为1.04915mg/L。在处理的幼虫中观察到行为改变,如兴奋、坐立不安、颤抖、痉挛然后瘫痪,说明这两种油可能对其神经肌肉系统产生了影响。显微观察处理幼虫的形态改变发现,与对照相比,大多数器官的外观正常,只是肛鳃略为内收缩而引起结构畸形,提示肛腮可能是这两种油的作用位点,腮的功能异常引起了幼虫死亡。这两种油品可开发用作防治蚊虫的新型杀幼虫药剂。
Pine oil and cinnamon oil are good adult repellents due to their aromatic odor, but little is known about their larvicidal effect on mosquitoes. In order to reveal the poisonous potential of commercially available Pinus longifolia oil and Cinnamomum zeylanicum cinnamon oil to the 4th instar larvae of Aedes aegypti originating from Delhi, India, we conducted an in-house study of larval mortality and behavioral changes and Morphological changes and other indicators to assess its larvicidal potential. The results showed that both oils had toxic effects on 4th instar larvae of Aedes aegypti, and the killing effect of pine oil was better than that of cinnamon oil. The LC50 and LC70 values of pine oil were 0.33093 mg / L and 0.54476 mg / L respectively, while the LC50 and LC70 values of Cassia oil were 0.63159 mg / L and 0.77736, respectively. Further observation showed that the larvicidal potential of cinnamon oil under LC90 was stronger than that of pine oil, with LC90 of 1.11879 mg / L and that of pine oil 1.04915 mg / L. Behavior changes, such as excitement, restlessness, trembling, convulsions and then paralysis were observed in the treated larvae, indicating that both oils may have an impact on their neuromuscular system. Microscopic observation of larval morphological changes found that compared with the control, the appearance of most organs is normal, but anal gills slightly contracted and cause structural deformity, suggesting that the anus may be the role of these two oil sites, the function of the gills Abnormalities cause larval death. Both oils can be developed as novel larvicidal agents for controlling mosquitoes.