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为了探究庆大霉素致原代肾小管上皮细胞的凋亡作用,以0、1、2、4、8、16 mmol/L浓度的庆大霉素分别作用原代大鼠肾小管上皮细胞24 h,采用cck-8法检测细胞相对活力,hochest 33258染色法观察细胞核形态以及利用流式细胞术检测细胞凋亡率。结果表明,与对照组相比,随着庆大霉素浓度的增加,细胞活性呈显著或极显著下降(P<0.05或P<0.01),细胞凋亡率呈显著或极显著上升(P<0.05或P<0.01),hochest33258染色观察到细胞核出现固缩或碎裂(P<0.01)。结果提示,庆大霉素能引起细胞发生凋亡和损伤,并且这可能与其肾毒性有密切的联系。
In order to investigate the apoptosis of primary renal tubular epithelial cells induced by gentamycin, primary rat renal tubular epithelial cells were treated with 0, 1, 2, 4, 8 and 16 mmol / L gentamicin respectively h, the relative viability of cells was detected by cck-8 method, the morphology of nucleus was observed by hochest 33258 staining, and the apoptosis rate was detected by flow cytometry. The results showed that compared with the control group, the cell viability was significantly or very significantly decreased with the increase of gentamicin concentration (P <0.05 or P <0.01), and the apoptosis rate was significantly or very significantly increased (P < 0.05 or P <0.01). Homozygous 33258 staining showed pyknosis or fragmentation of the nucleus (P <0.01). The results suggest that gentamicin can cause cell apoptosis and injury, and this may be closely related to its nephrotoxicity.