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目的探讨p57 kip2和细胞周期蛋白E在体外培养人子宫内膜癌JEC细胞中的表达,并分析雌二醇(E_2)对其的影响。方法分别采用低浓度(10~(-6)mol/L)、中浓度(10~(-8)mol/L)、高浓度(10~(-10)mol/L)的E_2对在体外培养的人子宫内膜癌JEC细胞进行处理,处理时间为1 d、2 d、3 d,处理完毕后采用Western Blot技术观察p57 kip2和细胞周期蛋白E的表达情况。结果Western Blot技术结果显示,JEC细胞经E_2处理1 d后,p57 kip2在高浓度组的表达水平最好,在中浓度组的表达水平最差,差异有统计学意义(P<0.01);细胞周期蛋白E在高浓度组的表达水平最差,在中浓度组的表达水平最好,差异有统计学意义(P<0.01)。JEC细胞经E_2处理2 d、3 d后,p57 kip2和细胞周期蛋白E的表达量随E_2处理浓度的升高而加大,差异均有统计学意义(均P<0.05)。p57 kip2和细胞周期蛋白E的表达互不影响(r=-0.01,P=1.02)。结论 p57 kip2和细胞周期蛋白E在EC发生、发展中的作用均受到E_2的影响。JEC细胞经E_2处理浓度越高及时间越长,其将由正常生长繁殖状态转变成生长繁殖抑制状态。
Objective To investigate the expression of p57 kip2 and cyclin E in human endometrial carcinoma JEC cells cultured in vitro and analyze the effect of estradiol (E_2) on it. Methods E_2 cultured at low concentration (10 -6 mol / L), medium concentration (10 -8 mol / L) and high concentration (10 -10 mol / L) Of human endometrial cancer JEC cells were treated for 1 d, 2 d, 3 d, Western blot was used to observe the expression of p57 kip2 and cyclin E after treatment. Results The results of Western Blot showed that the expression level of p57 kip2 was the highest in the high concentration group and the lowest in the middle concentration group (P <0.01) after 1 day of EEC treatment. The difference was statistically significant (P <0.01) The expression of cyclin E was the worst in the high concentration group and the highest in the middle concentration group, the difference was statistically significant (P <0.01). The expression of p57 kip2 and cyclin E increased with the increase of E 2 concentration in JEC cells treated with E 2 for 2 days and 3 days (all P <0.05). The expression of p57 kip2 and cyclin E did not affect each other (r = -0.01, P = 1.02). Conclusion The role of p57 kip2 and cyclin E in the development and progression of EC are influenced by E_2. JEC cells treated by E 2 at higher concentrations and longer time, it will change from the normal growth and reproduction to growth and reproduction inhibition state.