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目的 在大鼠肾移植急性排斥反应模型上观察移植肾组织的TGF -β1 的表达 ,对比观察青藤碱和亚治疗剂量的环孢菌素A对移植组织的TGF -β1 表达水平的影响 ,为寻求无肾毒免疫抑制剂治疗上的应用提供实验室依据。方法 建立 48个大鼠同种原位肾移植急性排斥反应模型 (Wistar SD) ,按施加的处理因素不同分为 4组 :对照组 (生理盐水 1ml·kg-1 ·d-1 ,ip)、CsA组 ( 2 5mg·kg-1 ·d-1 ,ip)、S +C组 (SIN3 0mg·kg-1 ·d-1 +2 5mg·kg-1 ·d-1 ,ip) ,每组 12只 ,随机抽取 6只观察大鼠移植肾受者存活时间 ,另 6只于术后第 6d处死 ,取移植肾组织标本行病理观察及免疫组织化学方法检测TGF -β1 表达并以BLTDMC -2 0 80超解析病理图文系统进行量化统计。分析SIN协同CsA对大鼠肾移植受者存活时间及对急性排斥反应时的肾组织TGF -β1 表达水平的影响。结果 对照组受体大鼠均在术后 9d以内死亡 ,平均生存 ( 7 83± 0 75 )d ,SIN( 3 0mg·kg-1 ·d-1 .ip)或CsA( 2 5mg·kg-1 ·d-1 .ip)可稍为延长受体鼠存活时间 ,SIN与CsA( 2 5mg·kg-1 ·d-1 .ip)联用后可明显延长受体鼠存活时间至 18d以上 ( 2 5 0 0± 4 65 )d ;移植肾组织的TGF -β1表达平均阳性面积单位在对照组为 ( 8 3 9± 0 67) %,SIN组为 ( 13 90?
Objective To observe the expression of TGF-β1 in rat renal allograft model after acute renal allograft rejection, and to compare the effect of sinomenine and sub-therapeutic cyclosporin A on the expression of TGF-β1 To provide a nephrotic immunosuppressive therapeutic application to provide laboratory evidence. Methods A total of 48 rats were randomly divided into 4 groups: control group (saline 1ml · kg-1 · d-1, ip), acute rejection model of allograft orthotopic kidney transplantation (Wistar SD) CsA group (25 mg · kg-1 · d-1, ip), S + C group (SIN3 0 mg · kg-1 · d-1 +2 5 mg · kg-1 · d-1, ip) Only 6 rats were randomly selected to observe the survival time of renal allograft recipients. The other 6 rats were sacrificed on the 6th day after operation. Transplanted renal allografts were examined by immunohistochemistry and histopathology. The expression of TGF-β1 was detected by BLTDMC-20 80 super-resolution pathological graphics system to quantify statistics. The effects of SIN in combination with CsA on the survival time of renal allograft recipients and the expression of TGF-β1 in renal tissues during acute rejection were analyzed. Results All rats in the control group died within 9 days after operation, with an average survival of 7 83 ± 0 75 d, SIN of 30 mg · kg-1 · d-1 · ip or CsA of 25 mg · kg-1 · D-1 .ip) could prolong the survival time of recipient mice. SIN and CsA (25mg · kg-1 · d-1ip) could significantly prolong the survival time of recipient mice to more than 18d 0 0 ± 4 65) d. The average positive expression area of TGF-β1 in the control group was (8 3 9 ± 0 67)% in the control group and (13 90 ± 0 67)