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目的调查山东省嘉祥县某中学发生的一起由腺病毒(Ad V)引发的呼吸道传染病疫情。方法结合现场流行病学和实验室检测等方法对该起呼吸道暴发疫情进行调查。采集发病者的咽拭子标本,利用15种常见呼吸道病毒的核酸检测套装对标本进行检测。确定病毒种类之后进行基因扩增并测序。在Gen Bank上与已知序列进行比对以确定其型别,并制作系统进化树。结果自2013年4月3日-7日,学校1 067名学生中共有12人发病。经PCR方法检测,4份Ad V基因为阳性。测序并比对后确定其全部为Ad V-7型。结论这次疫情由Ad V-7型引发,传播途径以呼吸道传播和密切接触为主,呼吸道症状相对较轻微。
Objective To investigate the epidemic situation of respiratory infection caused by adenovirus (Ad V) in a middle school in Jiaxiang County, Shandong Province. Methods The epidemic situation of respiratory tract was investigated by means of field epidemiology and laboratory tests. The throat swab samples were collected from the patients and the samples were detected by using 15 kinds of common respiratory virus nucleic acid test kits. After determining the type of virus, gene amplification and sequencing were performed. Compare with known sequences on Gen Bank to determine their type and make a phylogenetic tree. Results From April 3 to April 7, 2013, a total of 12 out of 1 067 students in the school were affected. According to the PCR method, four copies of Ad V gene were positive. Sequencing and alignment confirmed it was all Ad V-7. Conclusions The outbreak was caused by Ad V-7 and the main route of transmission was respiratory spread and close contact. Respiratory symptoms were relatively mild.