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抑制素(Inhibin)是一种性腺蛋白,已建立了放免测定法。本文详细研究了妊娠期血浆抑制素浓度。作者收集129例正常妊娠妇女的5~40孕周血浆样品,4例正常分娩后4天的产妇血浆标本和8例非孕妇女的滤泡中期与黄体中期血浆标本;从3例因妇科疾病而行子宫切除术的绝经前妇女获得黄体,从2例病人获得卵巢,胎盘绒毛叶从4例足月分娩妇女获得,所得组织经Eagle氏培养液清洗后进行匀浆、离心后收集上清液;从14例正常分娩婴儿的脐动脉和静脉分别取血;无抑制素血清从年龄大于55岁的绝经妇女而行卵巢切除术者获得。所有标本均贮于-20℃直至用放免法测定抑制素。
Inhibin is a gonadal protein, and a radioimmunoassay has been established. This article examines the plasma concentration of statins in pregnancy. The authors collected plasma samples from 5 to 40 weeks of gestation in 129 normal pregnant women, 4 maternal plasma samples from 4 normal postpartum births, and 8 medium and mid-luteal plasma samples from 8 non-pregnant women; from 3 patients with gynecological diseases The ovariectomized premenopausal women obtained corpus luteum from 2 patients. The placental villi were obtained from 4 full-term delivery women. The obtained tissue was washed with Eagle’s medium and then homogenized. The supernatant was collected after centrifugation. Blood was taken from the umbilical arteries and veins of 14 normal birth infants, and the statin-free sera was obtained from ovariectomized menopausal women over the age of 55 years. All specimens were stored at -20 ° C until inhibin was determined by radioimmunoassay.