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目的 探讨HBV -DNA定量在肝病发展及转归过程中的意义 ,以及乙型肝炎病毒标志物 (HBVM)与HBVDNA的相互关系。方法 血清的HBVM测定采用ELISA法 ,HBV -DNA定量利用外标法计算机辅助视频成像处理系统测定。结果 血清HBV -DNA含量随着HBsAg、HBeAg的转阴而下降。“小三阳”组HBVDNA( 1 .66× 1 0 9)显著低于“大三阳”组 ( 3.2 2× 1 0 9) ,P <0 .0 5。慢性肝炎 ,肝硬化 ,肝癌三者的血清HBV -DNA定量逐渐降低。且肝硬化组HBVDNA定量 ( 1 .1 5× 1 0 9)与慢性肝炎组 ( 1 .5 1× 1 0 9)差异非常显著 ,P <0 .0 0 1。结论 乙型肝炎病毒DNA是判断乙肝有否传染性的标准 ,HBV -DNA的定量测定可以准确地判断乙型肝炎患者的演变过程。e抗原转变为e抗体后病毒复制并未停止 ,而只是HBV -DNA复制的下降。肝病患者随着肝脏损害的不断加重 ,其HB -DNA的复制会降低。
Objective To investigate the significance of HBV-DNA quantification in the development and prognosis of liver disease and the relationship between hepatitis B virus markers (HBVM) and HBVDNA. Methods Serum HBVM was determined by ELISA and HBV-DNA quantification by computer-aided video imaging system using external standard method. Results Serum HBV-DNA levels decreased with negative HBsAg and HBeAg. HBVDNA (1.66 × 109) in “Sanyang” group was significantly lower than that in “Sanyangyang” group (3.2 2 × 109), P <0.05. Chronic hepatitis, cirrhosis, liver cancer serum HBV-DNA quantitatively decreased. The difference of HBVDNA in patients with cirrhosis (11.51 × 10 9) and chronic hepatitis (1.51 × 10 9) was significant (P <0.01). Conclusions Hepatitis B virus DNA is the standard to judge whether HBV is contagious. The quantitative determination of HBV-DNA can accurately judge the evolution of hepatitis B patients. Virus e antigen to e antibody replication did not stop, but only decreased HBV-DNA replication. Patients with liver disease as the liver damage continues to increase, its HB-DNA replication will be reduced.