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目的 测定细胞内穿孔素 (P + )及细胞表面抗原的表达 ,探讨其与移植肾急性排斥和长期存活的关系。方法 用流式细胞仪测定 2 2例患者外周血细胞毒T淋巴细胞和自然杀伤细胞亚群穿孔素的表达。结果 排斥组淋巴细胞穿孔素的表达高于非排斥组 ,排斥反应与CD4 +P+下调和CD8+P+上调有关。冲击治疗可引起P+T细胞亚群和CD5 6+P+NK细胞的降低。CD4 +P+及CD8+P+细胞的下调和CD5 6+P+上调在移植长期存活中多见。结论 观察外周血穿孔素的改变可以较早预测排斥反应 ,可作为评估移植肾长期存活的标志物。
Objective To determine the expression of intracellular perforin (P +) and cell surface antigens, and to explore its relationship with acute rejection and long-term survival of renal allografts. Methods Perforin expression in peripheral blood cytotoxic T lymphocytes and natural killer cell subsets in 22 patients was determined by flow cytometry. Results The expression of perforin in rejection group was higher than that in non-rejection group. The rejection was related to the down-regulation of CD4 + P + and the up-regulation of CD8 + P +. Impact therapy resulted in a decrease in P + T cell subsets and CD56 + P + NK cells. Down-regulation of CD4 + P + and CD8 + P + cells and upregulation of CD56 + P + are more common in long-term survival of the transplant. Conclusion Observing the changes of perforin in peripheral blood can predict the rejection earlier, which can be used as a marker to evaluate the long-term survival of transplanted kidney.