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目的研究凋亡抑制基因ｂｃ１－２的反义寡脱氧核苷酸（ＯＤＮ）对顺铂诱导肺癌细胞凋亡的促进作用。方法选择表达 ｂｅ１－２的肺癌细胞培养传代，分成 ７组：ＯＤＮ组、无意序列组、ＯＤＮ＋顺铂组、无意序列＋顺铂组、顺铂组、脂质体组和空白对照组。以脂质体与 ｂｃ１－２ＯＤＮ混合导入培养的相应组肺癌细胞中，培养６ ｈ后，相应孔加入抗癌药顺铂，再培养 １６ ｈ终止细胞培养并做细胞涂片，每组重复６个孔。用免疫细胞化学检测ｂｅ１－２的表达。采用末端脱氧核苷转移酶（ＴｄＴ）介导的脱氧尿苷三磷酸（ｄＵＴＰ）末端标记法（ＴＵＮＥＬ）原位观察癌细胞凋亡，并计算平均凋亡指数（ＡＩ）。结果经ＯＤＮ处理的癌细胞 ｂｃ１－２表达明显减弱，抑制有效率为 ６０．８％。凋亡的癌细胞胞核染成棕褐色。 ＯＤＮ＋顺铂组ＡＩ为１６．４±１．７，ＯＤＮ组ＡＩ为５．９±０．２，顺铂组ＡＩ为４．１±０．８，无意序列组ＡＩ为３．３±０．７，无意序列＋顺铂组ＡＩ为７．６±１．１，脂质体组ＡＩ为５．１±０．９，空白对照组ＡＩ为３．６±０．６。ＯＤＮ＋顺铂组ＡＩ明显高于其他各实验组（Ｐ＜０．０１）。ＯＤＮ组与顺铂组的ＡＩ之和明显小于ＯＤＮ＋顺铂组（Ｐ＜０．０１）。结论ｂｃ１－２反
Objective To study the effect of antisense oligodeoxynucleotide (ODN) of apoptotic suppressor gene bcl-2 on the apoptosis of lung cancer cells induced by cisplatin. METHODS: Lung cancer cells expressing be1-2 were selected and cultured and divided into 7 groups: ODN group, unintentional sequence group, ODN+cisplatin group, unintentional sequence+cisplatin group, cisplatin group, liposome group and blank control group. After the corresponding groups of lung cancer cells were mixed with liposome and bc1-2ODN, cultured for 6 h, corresponding anti-cancer drug cisplatin was added to corresponding wells, cultured for another 16 h to stop cell culture, and cell smears were made. Each group was repeated 6 times. hole. The expression of be1-2 was detected by immunocytochemistry. Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) terminal labeling (TUNEL) was used to observe the apoptosis of cancer cells in situ and calculate the average apoptotic index (AI). Results The expression of bcl-2 in ODN-treated cancer cells was significantly reduced, and the inhibition rate was 60.8%. The nucleus of the apoptotic cancer cells was browned. The AI in the ODN+cisplatin group was 16.4±1.7, the AI in the ODN group was 5.9±0.2, the AI in the cisplatin group was 4.1±0.8, and the unintentional sequence group AI was 3.3±0. 7. The AI of the unintentional sequence + cisplatin group was 7.6±1.1, the AI of the liposome group was 5.1±0.9, and the AI of the blank control group was 3.6±0.6. ODN + cisplatin group AI was significantly higher than other experimental groups (P <0.01). The sum of AI in the ODN group and the cisplatin group was significantly smaller than that in the ODN+cisplatin group (P<0.01). Conclusion bc1-2