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利用负压处理酶—愈伤组织或悬浮系的混合物,可使原生质体产量比各自对照提高2～3倍,并且可以降低酶使用浓度,缩短解离时间,促进生物酶的解离效率.实验证明,小麦愈伤组织或悬浮细胞在酶液中经700mmHg的负压下处理30分钟后就可使原生质体的产量大幅度提高.而且不会造成原生质体的破损和生活力的下降.培养7天后统计一次分裂频率,处理略高于对照.合适的负压处理时间在30～180分钟之间,具体应根据需要解离材料的来源和细胞状态而确定.结合负压处理,已成功地从有芒白7号成熟胚愈伤组织及悬浮系中分离出大量生活力高的原生质体,并得到细胞团和再生愈伤组织.植株的分化实验正在进行.
Using negative pressure to process the mixture of enzyme-callus or suspension, the protoplast yield can be increased by 2 to 3 times than that of the respective control, and the enzyme concentration can be decreased to shorten the dissociation time and promote the dissociation efficiency of the enzyme. Proved that wheat callus or suspension cells in the enzyme solution by 700mmHg under negative pressure for 30 minutes after the protoplast yield can be greatly improved without causing protoplast damage and decreased viability Culture 7 Day after the statistics of a cleavage frequency, the treatment slightly higher than the control.Appropriate negative pressure treatment time is 30 to 180 minutes, in particular, should be based on the source of dissociation material and cell state to determine the combination of negative pressure treatment has been successfully from A large number of protoplasts with high viability were isolated from the callus and suspension of Mature White embryos 7, and the cell mass and regenerated callus were obtained.The differentiation experiments of the plants were underway.