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人食管癌癌旁组织12例和正常食管粘膜15例组织块在盖玻上用199培养基培养,并掺入放射性氘胸腺嘧啶核苷.用光镜、显微分光光度计,放射自显影,扫描电镜检查移植块生长上皮层的生长速度,细胞核 DNA 含量,DNA 合成和形态学改变。结果是前三周,癌旁粘膜组织块培养上皮的生长率,细胞 DNA 含量和 DNA 合成高于食管正常粘膜.第四周以后两组上皮出现生长率降低,分化增加现象,在此培养中未能见到癌旁粘膜上皮转化为恶性细胞.本实验正常粘膜的培养,其生长的上皮细胞可以作为食管癌致癌因素研究的模型.
Twelve cases of human esophageal cancer adjacent tissues and 15 cases of normal esophageal mucosa were cultured on coverslips in 199 medium and incorporated with radioactive thymidine. Light microscopy, microspectrophotometry, and autoradiography were used. Scanning electron microscopy was performed to examine the growth rate, nuclear DNA content, DNA synthesis and morphological changes in the growth epithelium of the graft. As a result, in the first 3 weeks, the growth rate of the epithelium of the tissue adjacent to the tumor tissue, the cell DNA content and DNA synthesis was higher than that of the normal esophageal mucosa. After the fourth week, the growth rate decreased and the differentiation increased in the epithelium of the two groups. It can be seen that paraneoplastic epithelium turns into malignant cells. In this experiment, normal mucosal culture, the growth of epithelial cells can be used as a model for the study of esophageal cancer.