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目的 观察外源性溶血磷脂酰胆碱对离体大鼠工作心脏的损伤作用。方法 麻醉ＳＤ大鼠，取其心脏，进行Ｌａｎｇｅｎｄｏｒｆｆ 灌注，待心脏稳定后转为工作心脏状态。然后随机分为３ 组，对照组：正常灌注３５ｍｉｎ；外源性溶血磷脂酰胆碱组：用含５μｍｏｌ／ＬＬＰＣ的ＫＨ 液灌注５ｍｉｎ ，再用正常的ＫＨ 液灌注３０ｍｉｎ；缺血再灌注组：停灌注Ｋ－ Ｈ 液４０ｍｉｎ，再灌注３０ｍｉｎ 。结果 与对照组相比，外源性溶血磷脂酰胆碱组和缺血再灌注组的心功能指标明显降低，再灌后室颤发生率和灌注液中的乳酸脱氢酶明显升高，而且外源性溶血磷脂酰胆碱组和对照组相比没有明显的差异。结论 外源性溶血磷脂酰胆碱可导致类缺血再灌注损伤，其损伤程度与经典的方法基本接近。
Objective To observe the effect of exogenous lysophosphatidylcholine on the working heart of isolated rats. Methods SD rats were anesthetized and their hearts were perfused. Langendorff perfusion was performed and the hearts were stable and transferred to working heart condition. Then randomly divided into 3 groups, the control group: normal perfusion 35min; exogenous lysophosphatidylcholine group: with 5μmol / LLPC KH perfusion for 5min, and then normal KH perfusion 30min; ischemic reperfusion group: Stop perfusion K-H solution 40min, reperfusion 30min. Results Compared with the control group, the indexes of cardiac function in the exogenous lysophosphatidylcholine group and the ischemia-reperfusion group were significantly decreased. The incidence of ventricular fibrillation and the lactate dehydrogenase in the perfusion fluid were significantly increased after reperfusion, and There was no significant difference between the exogenous lysophosphatidylcholine group and the control group. Conclusion Exogenous lysophosphatidylcholine can cause ischemia-reperfusion injury and its damage is similar to that of the classic method.