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本实验使用从加拿大引种的自发性糖尿病大鼠,在生物学特性以及发病后的临床表现等方面进行观察。据生长发育、代谢、血液、生化与病理等指标表明,此种“BB”wistar系大鼠是一种典型的自发性Ⅰ型糖尿病动物模型。其发病时间为出生后50～213日(平均117日)。临床表现为,高血糖(200～794mg/dl)及酮尿,尿糖含量为20～270mg/dl,体重增长低于对照组(远交系Wistar大鼠),采食量与排尿量皆高于对照组(P<0.05)。光镜与电镜显示病鼠的肾小管上皮细胞内有糖原沉积;胰岛内β细胞大量破坏并减少,造成胰岛素分泌缺乏。大鼠发病后即注射精蛋白锌胰岛素,剂量按不同病情,以观察治疗效果。
In this experiment, spontaneous diabetic rats introduced from Canada were observed in terms of biological characteristics and clinical manifestations after the onset of disease. According to growth and development, metabolism, blood, biochemical and pathological indicators show that such “BB” wistar rats is a typical animal model of spontaneous type I diabetes. The onset time of 50 to 213 days after birth (average 117 days). Clinical manifestations, high blood sugar (200 ~ 794mg / dl) and ketone urine, urine sugar content of 20 ~ 270mg / dl, weight gain was lower than the control group (outbred Wistar rats), feed intake and urine output were high In the control group (P <0.05). Light and electron microscopy showed that the rat renal tubular epithelial cells have glycogen deposition; islet β cell damage and reduce a large number, resulting in a lack of insulin secretion. Rats after injection of protamine zinc insulin dose according to different conditions to observe the therapeutic effect.