本实验以布氏肉汤旋转培养法制备幽门螺杆菌提取液，观察其对胃粘膜细胞非程序ＤＮＡ合成的影响。结果表明幽门螺杆菌提取液可诱导胃粘膜细胞的ＵＤＳ。且在一定范围内呈剂量依赖关系。０～１０μｇ／ｍｌ的过氧化氢酶不诱细胞ＵＤＳ，对细胞也无毒性作用。２μｇ／ｍｌ的过氧化氢酶即可显著抑制幽门螺杆菌提取液诱导的胃粘膜细胞的ＵＤＳ，而失活的过氧化氢酶则无抑制作用。提示幽门螺杆菌提取液可引起胃粘膜细胞ＤＮＡ的损伤，而这种损伤可能与自由基的生成有关。 In this experiment, the Helicobacter pylori extract was prepared by the culture of Broth by Broth and the effect on the non-procedural DNA synthesis of gastric mucosal cells was observed. The results show that H. pylori extract can induce gastric mucosal UDS. And in a certain range in a dose-dependent manner. 0 ~ 10μg / ml of catalase does not induce cell UDS, no toxic effect on the cells. 2μg / ml of catalase can significantly inhibit the H. pylori-induced gastric mucosal UDS, while inactivated catalase has no inhibitory effect. Tip Helicobacter pylori extract can cause DNA damage in gastric mucosal cells, and this damage may be related to the formation of free radicals.