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采用宁杞１号枸杞的髓组织进行离体培养，诱导产生胚性愈伤组织，并用６０Ｃｏ－γ射线进行诱变，以枸杞根腐病病菌尖孢镰刀菌（ＦｕｓａｒｉｕｍｏｘｙｓｐｏｒｕｍＳｃｈｌｅｃｈｔ）菌株产生的致病培养液为选择剂，筛选抗病变异体，获得抗根腐病的愈伤组织。抗性愈伤组织经稳定性分析，其抗性稳定。抗性愈伤组织在含３０％毒素的培养基中可分化成再生植株，再生植株经过脯氨酸、叶绿素含量分析，二者含量都有所提高；经过过氧化物同功酶酶谱分析，变异体酶谱带数有所增加；变异体植株叶片经过根腐菌分生孢子液接种鉴定，再生植株抗病率达２４％，说明此变异体是抗根腐病的变异体
The medullary tissue of Ningqi No.1 medlar was used for in vitro culture to induce the embryogenic callus, and induced by 60 Co-γ ray, the pathogenic culture produced by the strain of Fusarium oxysporum Schlecht Liquid as a selection agent, screening disease-resistant variants to obtain callus resistant to root rot. Resistant callus by stability analysis, the resistance is stable. Resistant calli could be differentiated into regenerated plants in 30% toxin-containing medium. After proline and chlorophyll content of regenerated plants were analyzed, the content of both of them increased. After peroxidase isoenzyme analysis, Variant zymogram bands increased; Variant plant leaves after root rot fungi conidia identification, resistance rate of regenerated plants up to 24%, indicating that this variant is resistant to root rot variants