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目的观察乙醇对新生小鼠延髓基本节律性呼吸的作用。方法制作新生小鼠离体延髓脑片标本,包含面神经后核内侧区并保留舌下神经根,给予灌流改良Kreb′s液记录舌下神经根的节律性呼吸放电活动(RRDA)。实验分为2组:模型验证组使用改良Kreb′s液灌流脑片标本,记录舌下神经根的RRDA60 min,观察舌下神经根的RRDA有无改变,以判断实验模型的稳定性;实验组使用含0 mg.L-1(对照组)、200 mg.L-1(200 mg.L-1乙醇组)、800 mg.L-1(800 mg.L-1乙醇组)乙醇的改良Kreb′s液灌流脑片,每个浓度灌流15 min,对比RRDA改变,分析乙醇对延髓基本节律性呼吸作用。结果模型验证组舌下神经根的RRDA的呼吸周期、吸气时程、放电积分幅度等呼吸指标在60 min变化无统计学差异;200 mg.L-1的乙醇缩短呼吸周期、延长吸气时程、增加放电积分幅度,对RRDA有兴奋作用;800 mg.L-1的乙醇延长呼吸周期、缩短吸气时程、降低放电积分幅度,对RRDA有抑制作用。结论低质量浓度的乙醇对新生小鼠延髓基本节律性呼吸有兴奋作用,高质量浓度的乙醇对其有抑制作用。
Objective To observe the effect of ethanol on basic rhythmic breathing of medulla oblongata in neonatal mice. Methods Specimens of neonatal mouse isolated medulla oblongata were obtained. The medial area of the posterior nucleus of the facial nerve was preserved and the sublingual nerve roots were preserved. Rhythmic respiratory discharge activity (RRDA) was recorded by perfusion modified Kreb’s fluid. The experimental group was divided into two groups: the model validation group using modified Kreb’s liquid perfusion brain slice specimens, recording sublingual nerve root RRDA60 min, to observe the sublingual nerve root RRDA changes in order to determine the experimental model stability; experimental group Using modified Krebs containing 0 mg.L-1 (control), 200 mg.L-1 (200 mg.L-1 ethanol) and 800 mg.L-1 (800 mg.L-1 ethanol) ’S liquid perfusion brain slices, each concentration perfusion 15 min, compared with RRDA changes, the basic rhythm of ethanol on the rhythmic respiration. Results There was no significant difference in respiration period, inspiratory duration and discharge integral amplitude between RRDA group and control group at 60 min after model subcutaneous nerve root stimulation; ethanol at 200 mg.L-1 shortened respiratory cycle and prolonged inspiratory time Cheng, increase the magnitude of the discharge integral, RRDA excited; 800mg.L-1 ethanol to extend the respiratory cycle, shorten the inspiratory time, reduce the magnitude of the discharge integral, inhibition of RRDA. CONCLUSION: Low concentration of ethanol can stimulate basic rhythmic respiration of medulla oblongata in neonatal mice, and high concentration of ethanol can inhibit it.