目的:探讨嗜碱粒细胞是否可表达人类白细胞抗原(HLA)Ⅱ类分子.方法:采用密度梯度离心和免疫磁珠方法分离和纯化脐血嗜碱粒细胞,通过体外培养及粒细胞-巨噬细胞集落刺激因子(GM-CSF)和干扰素(IFN)-γ刺激20-60小时后,用流式细胞仪分析 HLA Ⅱ类分子表达的情况.结果:嗜碱粒细胞的纯度 ≥83.5％,在 GM-CSF10 μg/L或 IFN-γ100kU/L刺激后,其膜表面可表达HLA Ⅱ类分子,表达百分率在刺激后20小时较高,分别为10.2％±2.1％和11.3％±1.0％.结论:嗜碱粒细胞具有表达HLA Ⅱ类分子的潜在能力. Objective: To investigate whether basophil can express HLA class Ⅱ.Methods: Umbilical cord blood basophils were isolated and purified by density gradient centrifugation and immunomagnetic beads method, and cultured in vitro and granulocyte macrophage After stimulated with GM-CSF and IFN-γ for 20-60 hours, the expression of HLA class II molecules was analyzed by flow cytometry.Results The purity of basophils was 83.5% After stimulated with GM-CSF 10 μg / L or IFN-γ100 kU / L, HLA class II molecules were expressed on the membrane surface. The percentages of expression were higher at 20 hrs after stimulation, 10.2% ± 2.1% and 11.3% ± 1.0%, respectively. Conclusion: Basophils have the potential to express HLA class II molecules.