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本研究目的是探讨同一个体的牙龈和种植体周围粘膜与菌斑有关的损害特征。 材料和方法 研究对象为20例局部缺牙的志愿者(12女,8男,30～60岁)。牙周病治疗后,缺牙区采用种植修复。修复后6～24个月,切取同颌内的天然牙龈组织(gingival tissue,GM)和靠近种植体周粘膜(periimplant mucosa,PIM)进行活检。标本平分为二组。一组经固定包埋染色后,作组织学和形态学分析;另一组经液氮速冻切片后采用免疫组化法行截面分析,确定炎性细胞浸润区面积;评估不同细胞和细胞标志物的
The purpose of this study was to investigate the lesion characteristics associated with plaque around the gums and implants of the same individual. Materials and Methods The study population was 20 volunteers with partial edentulous teeth (12 female, 8 male, 30-60 years old). Periodontal disease treatment, edentulous area using implant repair. Six months to 24 months after repair, the gingival tissue (GM) in the same jaw and the periimplant mucosa (PIM) were dissected for biopsy. The specimens were divided into two groups. One group was fixed with embedded staining for histological and morphological analysis; the other group was frozen section after liquid nitrogen immunohistochemistry cross-section analysis to determine the area of inflammatory cell infiltration area; assessment of different cell and cell markers of