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目的:观察慢性间歇性低压低氧(chronic intermittent hypobaric hypoxia,CIHH)模型大鼠下丘脑OrexinA表达及舌下神经放电活动,探讨CIHH对呼吸活动调节的机制。方法:对正常成年雄性SD大鼠进行每天6 h的低压低氧处理,28 d后进行血气分析,采用免疫组织化学染色方法观察大鼠下丘脑Orexin A的表达,记录舌下神经放电作为观察呼吸活动的指标。结果:血气分析显示模型组大鼠的PO2下降,PCO2明显升高(P<0.05,n=6);模型组大鼠下丘脑Orexin A免疫阳性神经元的相对光密度值显著升高(P<0.01,n=5);舌下神经放电幅度积分面积减小(P<0.01,n=6),呼吸频率降低(P<0.05,n=6)。结论:CIHH使大鼠动脉血PO2下降、PCO2明显升高,舌下神经放电活动减弱。下丘脑Orexin A神经元表达的增加提示Orexin的确参与了对呼吸活动的调节,尤其在CIHH时发挥了重要作用。
Objective: To observe the hypothalamic OrexinA expression and hypoglossal nerve discharge activity in chronic intermittent hypobaric hypoxia (CIHH) model rats and to explore the mechanism of CIHH regulating respiratory activity. Methods: Normal adult male Sprague-Dawley rats were treated with hypobaric hypoxia for 6 hours every day. Blood gas analysis was performed 28 days later. The expression of Orexin A in hypothalamus was observed by immunohistochemical staining. The hypoglossal nerve discharge was recorded as observation respiration Activity indicators. Results: Blood gas analysis showed that the PO2 of model rats decreased and PCO2 increased significantly (P <0.05, n = 6). The relative optical density of Orexin A immunoreactive neurons in hypothalamus of model rats increased significantly (P < 0.01, n = 5). The area of hypoglossal nerve discharge decreased (P <0.01, n = 6) and respiratory rate decreased (P <0.05, n = 6). Conclusion: CIHH decreased the PO2 of arterial blood in rats, and the PCO2 was significantly increased, and the activities of hypoglossal nerve discharge were weakened. Increased expression of hypothalamic Orexin A neurons suggests that Orexin is indeed involved in the regulation of respiratory activity, especially in CIHH.