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目的总结一步法抽提人体肝细胞癌(HCC)癌组织及实验性肝纤维化肝组织总RNA的体会。方法参照Chomczynski一步法抽提12例人体HCC癌组织及16例大鼠纤维化肝组织总RNA。结果及体会 28例标本一次性抽提成功,且得率较高。本研究体会到,保持标本新鲜,少坏死,避免内源性RNA酶(RNase)活化及外源性RNase污染是一次性抽提成功的关键。
Objective To summarize the experience of one-step extraction of total RNA from human hepatocellular carcinoma (HCC) and experimental liver fibrosis. Methods Chomczynski reference to one-step extraction of 12 cases of human HCC cancer tissue and 16 cases of fibrosis in rats liver total RNA. Results and Experience 28 cases of specimens were successfully extracted at one time, and the yield was high. This study has realized that keeping samples fresh, less necrotic, avoiding RNase activation and exogenous RNase contamination are the keys to successful one-time extraction.