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为研究可表达微血管密度的肿瘤血管生成强度能否作为Ⅰ_B和Ⅱ_A期宫颈癌预示淋巴结转移和生存参数,对114例Ⅰ_B和Ⅱ_A期宫颈癌的活检和子宫切除组织进行常规石蜡包埋,标准组化技术染色,确定肿瘤分级和淋巴血管受侵情况。使用标准免疫过氧化物酶技术染内皮细胞以显示血管,根据Weidner等方法定量微血管,即在低放大区域(×40和×100)检查切片中血管密集区,然后通过计算机图像分析计数区所有血管(×250),定义为所有棕染内皮细胞或内皮细胞堆集,无论有无管腔形成,并与邻近微血管、肿瘤细胞,或其它结缔组织成分鉴别。由计算机决定测量区域,至少计算8～10个视野,测量平
To investigate whether the intensity of tumor angiogenesis that can express microvessel density can be used as a predictor of lymph node metastasis and survival in stage I-B and II-A cervical cancer, routine paraffin embedding was performed on 114 patients with stage I-B and II-A cervical cancer biopsy and hysterectomy. The staining technique was used to determine tumor grade and lymphatic invasion. Endothelial cells were stained with standard immunoperoxidase technology to visualize blood vessels. Microvessels were quantified according to the method of Weidner et al., ie, blood vessel-intensive regions were examined in the low-amplification region (×40 and ×100), and all blood vessels in the count region were analyzed by computer images. (×250), defined as the accumulation of all brown-stained endothelial cells or endothelial cells, with or without lumen formation, and identification with adjacent microvessels, tumor cells, or other connective tissue components. Determine the measurement area by computer, calculate at least 8 to 10 fields of view, measure the level